pHAGE PGK-GFP-IRES-LUC-W

LOCUS       pHAGE PGK-GFP-IRES-LUC-W                9272 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  2nd generation lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    pHAGE PGK-GFP-IRES-LUC-W
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9272)
  AUTHORS   Wilson AA, Murphy GJ, Hamakawa H, Kwok LW, Srinivasan S, Hovav AH, 
            Mulligan RC, Amar S, Suki B, Kotton DN
  TITLE     Amelioration of emphysema in mice through lentiviral transduction of
            long-lived pulmonary alveolar macrophages.
  JOURNAL   J Clin Invest. 2010 Jan . 120(1):379-89.
  PUBMED    20038801
REFERENCE   2  (bases 1 to 9272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9272
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(2261..2284)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2611..2630
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      2686..2695
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2692..3411
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2737..2758)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2998..3017)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3345..3366
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3415..3982
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3432..3984
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3576..3593)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3803..3822
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3996..5648
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(4062..4080)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     misc_feature    5662..6250
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5715..5735)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6133..6144)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6325..6558
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6677..6695)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6763..6867
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6868..7728
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7086..7105)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7899..8487
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8388..8407
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8641..8658
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     8750..8769
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     protein_bind    8898..8919
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8934..8964
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8972..8988
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8977..8999
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8996..9012
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8996..9012
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9062..9081
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagcatt
     2221 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc
     2281 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg
     2341 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct
     2401 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt
     2461 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg
     2521 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc aggggcgggg
     2581 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     2641 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcggccgc catggtgagc
     2701 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     2761 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     2821 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     2881 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     2941 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     3001 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     3061 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     3121 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     3181 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     3241 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     3301 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     3361 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta aggatccctc
     3421 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
     3481 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
     3541 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
     3601 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
     3661 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
     3721 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
     3781 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
     3841 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac
     3901 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
     3961 cctttgaaaa acacgatgat aatatggcca cacatatgga agacgccaaa aacataaaga
     4021 aaggcccggc gccattctat ccgctggaag atggaaccgc tggagagcaa ctgcataagg
     4081 ctatgaagag atacgccctg gttcctggaa caattgcttt tacagatgca catatcgagg
     4141 tggacatcac ttacgctgag tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac
     4201 gatatgggct gaatacaaat cacagaatcg tcgtatgcag tgaaaactct cttcaattct
     4261 ttatgccggt gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg aacgacattt
     4321 ataatgaacg tgaattgctc aacagtatgg gcatttcgca gcctaccgtg gtgttcgttt
     4381 ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa gctcccaatc atccaaaaaa
     4441 ttattatcat ggattctaaa acggattacc agggatttca gtcgatgtac acgttcgtca
     4501 catctcatct acctcccggt tttaatgaat acgattttgt gccagagtcc ttcgataggg
     4561 acaagacaat tgcactgatc atgaactcct ctggatctac tggtctgcct aaaggtgtcg
     4621 ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc cagagatcct atttttggca
     4681 atcaaatcat tccggatact gcgattttaa gtgttgttcc attccatcac ggttttggaa
     4741 tgtttactac actcggatat ttgatatgtg gatttcgagt cgtcttaatg tatagatttg
     4801 aagaagagct gtttctgagg agccttcagg attacaagat tcaaagtgcg ctgctggtgc
     4861 caaccctatt ctccttcttc gccaaaagca ctctgattga caaatacgat ttatctaatt
     4921 tacacgaaat tgcttctggt ggcgctcccc tctctaagga agtcggggaa gcggttgcca
     4981 agaggttcca tctgccaggt atcaggcaag gatatgggct cactgagact acatcagcta
     5041 ttctgattac acccgagggg gatgataaac cgggcgcggt cggtaaagtt gttccatttt
     5101 ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct gggcgttaat caaagaggcg
     5161 aactgtgtgt gagaggtcct atgattatgt ccggttatgt aaacaatccg gaagcgacca
     5221 acgccttgat tgacaaggat ggatggctac attctggaga catagcttac tgggacgaag
     5281 acgaacactt cttcatcgtt gaccgcctga agtctctgat taagtacaaa ggctatcagg
     5341 tggctcccgc tgaattggaa tccatcttgc tccaacaccc caacatcttc gacgcaggtg
     5401 tcgcaggtct tcccgacgat gacgccggtg aacttcccgc cgccgttgtt gttttggagc
     5461 acggaaagac gatgacggaa aaagagatcg tggattacgt cgccagtcaa gtaacaaccg
     5521 cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt accgaaaggt cttaccggaa
     5581 aactcgacgc aagaaaaatc agagagatcc tcataaaggc caagaagggc ggaaagatcg
     5641 ccgtgtaaat cgatagatcc taatcaacct ctggattaca aaatttgtga aagattgact
     5701 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     5761 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     5821 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     5881 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     5941 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     6001 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     6061 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     6121 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     6181 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     6241 gcctccccgc ctgagatcct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     6301 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     6361 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     6421 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     6481 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     6541 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     6601 caaagaaatg aatatcagag agtgagaggc ccgggttaat taaggaaagg gctagatcat
     6661 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     6721 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     6781 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     6841 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     6901 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     6961 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     7021 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     7081 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     7141 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     7201 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     7261 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     7321 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     7381 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     7441 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     7501 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     7561 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     7621 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     7681 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     7741 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     7801 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     7861 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     7921 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     7981 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     8041 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     8101 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     8161 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     8221 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     8281 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     8341 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     8401 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     8461 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     8521 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     8581 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     8641 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     8701 cgttggccga ttcattaatg cagcaagctc atggctgact aatttttttt atttatgcag
     8761 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     8821 gcctaggctt ttgcaaaaag ctccccgtgg cacgacaggt ttcccgactg gaaagcgggc
     8881 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     8941 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     9001 aacagctatg acatgattac gaatttcaca aataaagcat ttttttcact gcattctagt
     9061 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
     9121 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg
     9181 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt
     9241 atttgttaaa tatgtactac aaacttagta gt
//